Hotspot

rps16

LSC · 5277-6417 · 1141 bp

Back to Pentactina

Pi 0.0105

MarkerSeek score 50.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1141
variable_sites: 12
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAGTTCCACCGAAAACCAC	AACAACCCCAATCTTCACCC	50.0	50.0	58.0	58.0	1981-1988 bp	1.0	81.6
rps16_p2	ACTTGTGTTGGATTGGCACT	AACAACCCCAATCTTCACCC	45.0	50.0	57.9	58.0	2133-2144 bp	1.0	81.4
rps16_p3	AGAGTTCCACCGAAAACCAC	CCCCAATCTTCACCCTTTCC	50.0	55.0	58.0	58.1	1976-1983 bp	1.0	80.5
rps16_p4	ACTTGTGTTGGATTGGCACT	CCCCAATCTTCACCCTTTCC	45.0	55.0	57.9	58.1	2128-2139 bp	1.0	80.3
rps16_p5	AGAGTTCCACCGAAAACCAC	CAACCCCAATCTTCACCCTT	50.0	50.0	58.0	57.7	1979-1986 bp	1.0	79.2