Hotspot

psbE-petL

LSC · 65930-67293 · 1364 bp

Back to Pentactina

Pi 0.0138

MarkerSeek score 69.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1364
variable_sites: 18
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1429-1479 bp	1.0	81.6
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.2	58.2	1515-1565 bp	1.0	81.2
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	55.0	55.0	58.2	58.2	1424-1474 bp	1.0	81.2
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCG	50.0	55.0	58.2	58.2	1520-1570 bp	1.0	81.1
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.8	58.3	1428-1478 bp	1.0	80.9