Hotspot

atpF-atpH

LSC · 13591-14060 · 470 bp

Back to Pentactina

Pi 0.00907

MarkerSeek score 65.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 4
pi: 0.00907
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	623-652 bp	1.0	77.7
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	624-653 bp	1.0	77.6
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	625-654 bp	1.0	77.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	627-656 bp	1.0	77.5
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CAGAGGCGGAGGGAAAAATA	50.0	50.0	57.9	57.3	629-658 bp	1.0	77.5