Hotspot

trnK-UUU-rps16

LSC · 4268-5276 · 1009 bp

Back to Pentactina

Pi 0.0164
MarkerSeek score 58.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1009
variable_sites
15
pi
0.0164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1191-1251 bp 1.0 84.8
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG CGACGAGAGGGAAGAGATCT 55.0 55.0 58.0 58.0 1285-1345 bp 1.0 84.6
trnK-UUU-rps16_p3 ACCGTAGTTAGGGCTCTACC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1357-1417 bp 1.0 84.4
trnK-UUU-rps16_p4 TCAGGATCAGTCGTGGTCTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 1187-1247 bp 1.0 84.3
trnK-UUU-rps16_p5 TTCAGGATCAGTCGTGGTCT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 1188-1248 bp 1.0 84.3