Hotspot

trnH-GUG-psbA

LSC · 119-394 · 276 bp

Back to Pentactina

Pi 0.0388
MarkerSeek score 70.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
276
variable_sites
10
pi
0.0388
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 392-401 bp 1.0 84.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 406-415 bp 1.0 84.1
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 405-414 bp 1.0 84.1
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 407-416 bp 1.0 84.1
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 331-340 bp 1.0 83.1