Hotspot

psaC

SSC · 121835-122080 · 246 bp

Back to Pentaclethra

Pi 0.493

MarkerSeek score 76.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 112
pi: 0.493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TTTCTTTCCACCCCACATGG	ACCGGCTATTGTTTCGTCAA	50.0	45.0	58.0	57.8	1192 bp	0.5	58.6
psaC_p2	CCCGTCAGTAAAATAGGGCC	ACCGGCTATTGTTTCGTCAA	55.0	45.0	58.0	57.8	996 bp	0.5	58.3
psaC_p3	TTTCTTTCCACCCCACATGG	GCTATTGGACCGGCTATTGT	50.0	50.0	58.0	57.7	1200 bp	0.5	57.9
psaC_p4	TTTCTTTCCACCCCACATGG	GACCGGCTATTGTTTCGTCA	50.0	50.0	58.0	58.3	1193 bp	0.5	57.7
psaC_p5	CCCGTCAGTAAAATAGGGCC	GCTATTGGACCGGCTATTGT	55.0	50.0	58.0	57.7	1004 bp	0.5	57.7