Hotspot

ndhF

SSC · 115038-117275 · 2238 bp

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Pi 0.465

MarkerSeek score 79.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 902
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTTTCGATGCAACAACCAA	TCAAAAGAGATCAAATTGGCGA	40.0	36.4	56.8	56.6	2827 bp	0.5	23.1
ndhF_p2	ATGTTTCGATGCAACAACCA	TCAAAAGAGATCAAATTGGCGA	40.0	36.4	56.5	56.6	2828 bp	0.5	21.3
ndhF_p3	TGTTTCGATGCAACAACCAA	TCAAAAGAGATCAAATTGGCGAA	40.0	34.8	56.8	57.3	2827 bp	0.5	20.5
ndhF_p4	TGTTTCGATGCAACAACCAA	TTCAAAAGAGATCAAATTGGCGA	40.0	34.8	56.8	57.3	2828 bp	0.5	20.5
ndhF_p5	TGTTTCGATGCAACAACCAA	TGGCGAATAAGAAACAAAACTCA	40.0	34.8	56.8	57.2	2811 bp	0.5	20.0