Hotspot

ndhD

SSC · 120213-121718 · 1506 bp

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Pi 0.475

MarkerSeek score 80.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 611
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AATGAGGCCTGGGGATCATA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	2270 bp	0.5	58.8
ndhD_p2	TAATGAGGCCTGGGGATCAT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	2271 bp	0.5	58.8
ndhD_p3	TGAGGCCTGGGGATCATATT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	2268 bp	0.5	58.8
ndhD_p4	AATGAGGCCTGGGGATCATA	AAACAACTCGAAGCATGGGT	50.0	45.0	57.9	58.0	2176 bp	0.5	58.7
ndhD_p5	TGAGGCCTGGGGATCATATT	AAACAACTCGAAGCATGGGT	50.0	45.0	57.9	58.0	2174 bp	0.5	58.7