Hotspot

rps15-ycf1

SSC · 128414-128807 · 394 bp

Back to Pentaclethra

Pi 0.438

MarkerSeek score 77.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 152
pi: 0.438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAATCGATCCCGGGTTTTC	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.0	58.0	518 bp	0.5	59.6
rps15-ycf1_p2	CAATCGATCCCGGGTTTTCT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.0	58.0	517 bp	0.5	59.5
rps15-ycf1_p3	TCCTAACTGTTCCGGTCCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.1	58.0	1090 bp	0.5	59.4
rps15-ycf1_p4	AACACCATGCATTGATGGGT	GCAGCCGTTTCAGTATGGTA	45.0	50.0	58.1	58.0	895 bp	0.5	59.4
rps15-ycf1_p5	CTTGTAGCTGGGGCATTCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	57.9	58.0	840 bp	0.5	59.3