Hotspot

ndhG-ndhI

SSC · 123425-123850 · 426 bp

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Pi 0.436

MarkerSeek score 77.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 426
variable_sites: 159
pi: 0.436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CAAGAACCAGTCCCAACGAA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	631 bp	0.5	58.9
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	621 bp	0.5	58.7
ndhG-ndhI_p3	CCCAACGGTCCAAAGATGAA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.1	57.9	789 bp	0.5	58.5
ndhG-ndhI_p4	TTGCACCTATCAAAGCAGCT	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.8	57.9	1012 bp	0.5	57.6
ndhG-ndhI_p5	CAAGAACCAGTCCCAACGAA	TTTGGGCCGTTTACCAATGT	50.0	45.0	58.0	58.3	620 bp	0.5	57.3