Hotspot

ndhD-psaC

SSC · 121719-121834 · 116 bp

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Pi 0.595

MarkerSeek score 76.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 66
pi: 0.595
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTCTTTCCACCCCACATGG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	728 bp	0.5	59.8
ndhD-psaC_p2	TTTCTTTCCACCCCACATGG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.0	58.0	634 bp	0.5	59.8
ndhD-psaC_p3	TAATCGGCGATTTGACAGCA	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	933 bp	0.5	59.7
ndhD-psaC_p4	TAATCGGCGATTTGACAGCA	AAACAACTCGAAGCATGGGT	45.0	45.0	58.0	58.0	839 bp	0.5	59.7
ndhD-psaC_p5	TTTCTTTCCACCCCACATGG	GGCATGAAACAACTCGAAGC	50.0	50.0	58.0	58.0	640 bp	0.5	59.7