Hotspot

ycf1-fragment

SSC · 112645-118209 · 5565 bp

Back to Penstemon

Pi 0.0129

MarkerSeek score 53.80

Primer available yes

Label ycf1-fragment

Feature Metadata

label_name: ycf1-fragment
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 107
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-fragment_p1	TCTTTTCCATCACTTTGGCA	TAGCCCCGTTTCTGAAGAGT	40.0	50.0	55.4	58.4	252 bp	1.0	59.6
ycf1-fragment_p2	TTCTTTTCCATCACTTTGGCA	TAGCCCCGTTTCTGAAGAGT	38.1	50.0	56.3	58.4	253 bp	1.0	58.0
ycf1-fragment_p3	TCTTTTCCATCACTTTGGCAT	TAGCCCCGTTTCTGAAGAGT	38.1	50.0	56.0	58.4	252 bp	1.0	56.2
ycf1-fragment_p4	TTTCTTTTCCATCACTTTGGCA	TAGCCCCGTTTCTGAAGAGT	36.4	50.0	57.0	58.4	254 bp	1.0	55.8
ycf1-fragment_p5	TCTTTTCCATCACTTTGGCA	CCCCGTTTCTGAAGAGTCTT	40.0	50.0	55.4	57.2	249 bp	1.0	55.7