Hotspot

rps19

IRa · 142676-142724 · 49 bp

Back to Penstemon

Pi 0.062

MarkerSeek score 66.50

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRa
length_bp: 49
variable_sites: 3
pi: 0.062
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGGGTAGAGCCGGATCTAAG	CGTAGCCGCTCATGGTTATT	55.0	50.0	58.0	58.1	976-983 bp	1.0	84.9
rps19_p2	TGGGTAGAGCCGGATCTAAG	TGGATCAAGGCAGTGGATTG	55.0	50.0	58.0	57.9	394-428 bp	1.0	83.2
rps19_p3	TGGGTAGAGCCGGATCTAAG	GGATCAAGGCAGTGGATTGT	55.0	50.0	58.0	57.9	393-427 bp	1.0	83.2
rps19_p4	TGGGTAGAGCCGGATCTAAG	CGGATGTAGCCAAGTGGATC	55.0	55.0	58.0	58.1	408-417 bp	0.666667	67.9
rps19_p5	TGGGTAGAGCCGGATCTAAG	GGCCTGTAGTGGGTATTTGG	55.0	55.0	58.0	57.9	891 bp	0.333333	52.9