Hotspot

atpF

LSC · 1586-2851 · 1266 bp

Back to Penstemon

Pi 0.00528

MarkerSeek score 49.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1266
variable_sites: 10
pi: 0.00528
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CAATACCATCGCCCACTTGA	GCTGTAGAAGGTATCGCGAG	50.0	55.0	58.0	58.0	1960-1964 bp	1.0	80.9
atpF_p2	CACCCGCCATTACTTCATCA	GCTGTAGAAGGTATCGCGAG	50.0	55.0	58.0	58.0	1996-2000 bp	1.0	80.9
atpF_p3	CCGTTTCGGCTACCACAATA	GCTGTAGAAGGTATCGCGAG	50.0	55.0	58.0	58.0	2527-2531 bp	1.0	80.8
atpF_p4	ATTCGACTAATTCACCCGCC	GCTGTAGAAGGTATCGCGAG	50.0	55.0	58.1	58.0	2008-2012 bp	1.0	80.8
atpF_p5	CCGTTTCGGCTACCACAATA	GCCTTGGACTTGTCATTTGC	50.0	50.0	58.0	57.9	2339-2343 bp	1.0	80.7