Hotspot

psbK-psbI

LSC · 150228-150627 · 400 bp

Back to Penstemon

Pi 0.0368

MarkerSeek score 68.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 21
pi: 0.0368
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	511-520 bp	1.0	84.0
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	512-521 bp	1.0	84.0
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	516-525 bp	1.0	83.9
psbK-psbI_p4	CTTTTGTTTGGCAAGCTGCT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	517-526 bp	1.0	83.9
psbK-psbI_p5	CTTTTGTTTGGCAAGCTGCT	TTACGCCCCGGATCATTAGA	45.0	50.0	58.1	58.3	518-527 bp	1.0	82.1