Hotspot

rps16-trnQ-UUG

LSC · 148771-149610 · 840 bp

Back to Penstemon

Pi 0.0188

MarkerSeek score 63.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 23
pi: 0.0188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	971-981 bp	1.0	81.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	913-923 bp	1.0	81.0
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.4	58.0	985-995 bp	1.0	79.5
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	927-937 bp	1.0	79.1
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.2	58.0	963-973 bp	1.0	76.2