Hotspot

trnK-UUU-rps16

LSC · 146912-147589 · 678 bp

Back to Penstemon

Pi 0.0181
MarkerSeek score 63.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
678
variable_sites
18
pi
0.0181
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 788-801 bp 1.0 68.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 787-800 bp 1.0 68.5
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC GTGCTCAACCCACAGGAAC 50.0 57.9 58.3 58.7 802-815 bp 1.0 67.7
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 787-800 bp 1.0 67.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 786-799 bp 1.0 67.7