Hotspot

rpl2-rps19

IRa · 142613-142675 · 63 bp

Back to Penstemon

Pi 0.0328
MarkerSeek score 64.00
Primer available yes
Label rpl2-rps19

Feature Metadata

label_name
rpl2-rps19
feature_type
igs
region
IRa
length_bp
63
variable_sites
3
pi
0.0328
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rps19_p1 GATTGTTCGTCGCCGTAGTA TTGGCTACAAAAGGGTTTTT 50.0 35.0 57.8 53.7 124-126 bp 0.666667 33.7
rpl2-rps19_p2 TTTGATTGTTCGTCGCCGTA TTGGCTACAAAAGGGTTTTT 45.0 35.0 58.2 53.7 127-129 bp 0.666667 33.4
rpl2-rps19_p3 AATTTGATTGTTCGTCGCCG TTGGCTACAAAAGGGTTTTT 45.0 35.0 57.8 53.7 129-131 bp 0.666667 33.1
rpl2-rps19_p4 CACAACCCCTTGGGGTTATC TTGGCTACAAAAGGGTTTTT 55.0 35.0 58.2 53.7 193-195 bp 0.666667 33.0
rpl2-rps19_p5 TTGGGGTTATCCTGCACTTG TTGGCTACAAAAGGGTTTTT 50.0 35.0 57.8 53.7 184-186 bp 0.666667 32.6