Hotspot

ndhD-psaC

SSC · 105934-106014 · 81 bp

Back to Penstemon

Pi 0.0542

MarkerSeek score 71.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 81
variable_sites: 6
pi: 0.0542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GCAAGGAAAATGATTCGTGGT	CGCAGTATGGGTCTAGCTTA	42.9	50.0	57.4	56.5	148-150 bp	1.0	60.5
ndhD-psaC_p2	AGCAAGGAAAATGATTCGTGG	CGCAGTATGGGTCTAGCTTA	42.9	50.0	57.1	56.5	149-151 bp	1.0	58.2
ndhD-psaC_p3	GCAAGGAAAATGATTCGTGGT	CGCAGTATGGGTCTAGCTTAT	42.9	47.6	57.4	57.1	148-150 bp	1.0	56.7
ndhD-psaC_p4	AAGCAAGGAAAATGATTCGTGG	CGCAGTATGGGTCTAGCTTA	40.9	50.0	57.8	56.5	150-152 bp	1.0	55.6
ndhD-psaC_p5	AGCAAGGAAAATGATTCGTGG	CGCAGTATGGGTCTAGCTTAT	42.9	47.6	57.1	57.1	149-151 bp	1.0	54.4