Hotspot

psbH-petB

LSC · 64511-65515 · 1005 bp

Back to Penstemon

Pi 0.00966

MarkerSeek score 63.40

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 14
pi: 0.00966
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	ATACTTGCCGACCATCGATG	50.0	50.0	57.9	57.8	1201-1211 bp	1.0	79.5
psbH-petB_p2	GTCGCAATGGCTCTATTTGC	ATCATACTTGCCGACCATCG	50.0	50.0	57.9	57.8	1204-1214 bp	1.0	79.5
psbH-petB_p3	GTCGCAATGGCTCTATTTGC	CATACTTGCCGACCATCGAT	50.0	50.0	57.9	57.8	1202-1212 bp	1.0	79.5
psbH-petB_p4	AACTCCTTTGATGGGTGTCG	ATACTTGCCGACCATCGATG	50.0	50.0	57.8	57.8	1217-1227 bp	1.0	78.9
psbH-petB_p5	AACTCCTTTGATGGGTGTCG	CATACTTGCCGACCATCGAT	50.0	50.0	57.8	57.8	1218-1228 bp	1.0	78.9