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psbT-psbN

LSC · 63982-64048 · 67 bp

Back to Penstemon

Pi 0.0498

MarkerSeek score 68.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 5
pi: 0.0498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	133 bp	1.0	68.4
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	47.6	50.0	57.6	57.4	134 bp	1.0	67.1
psbT-psbN_p3	TCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	47.6	55.0	57.3	58.4	134 bp	1.0	66.2
psbT-psbN_p4	TCGAGAACCACCTAAAGTTCC	AGATCCATTCGAGGAACACG	47.6	50.0	57.3	57.4	135 bp	1.0	64.9
psbT-psbN_p5	TTCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	45.5	55.0	58.0	58.4	135 bp	1.0	63.5