Hotspot

trnT-UGU-trnL-UAA

LSC · 36605-37275 · 671 bp

Back to Penstemon

Pi 0.0119

MarkerSeek score 64.70

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 12
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	733-750 bp	1.0	78.8
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	TCATTCCGGGGTTTCTCTGA	55.0	50.0	57.9	58.3	807-824 bp	1.0	77.8
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	765-782 bp	1.0	77.8
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	727-744 bp	1.0	77.5
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	TCATTCCGGGGTTTCTCTGA	50.0	50.0	57.8	58.3	839-856 bp	1.0	76.9