Hotspot

ycf1

SSC · 126214-129813 · 3600 bp

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Pi 0.0114

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3600
variable_sites: 77
pi: 0.0114
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAAGTGGGGGAATGTGCAT	TCTTACTCTTACGATTTGGTT	45.0	33.3	58.0	52.2	247 bp	1.0	42.6
ycf1_p2	AAAGTGGGGGAATGTGCATT	TCTTACTCTTACGATTTGGTT	45.0	33.3	58.0	52.2	246 bp	1.0	42.6
ycf1_p3	AAAGTGGGGGAATGTGCATT	TTCTTACTCTTACGATTTGGT	45.0	33.3	58.0	52.2	247 bp	1.0	42.6
ycf1_p4	AAGTGGGGGAATGTGCATTT	TCTTACTCTTACGATTTGGTT	45.0	33.3	58.0	52.2	245 bp	1.0	42.6
ycf1_p5	AAGTGGGGGAATGTGCATTT	TTCTTACTCTTACGATTTGGT	45.0	33.3	58.0	52.2	246 bp	1.0	42.6