Hotspot

ccsA

SSC · 114922-115890 · 969 bp

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Pi 0.00681

MarkerSeek score 43.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 12
pi: 0.00681
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAGTGAAATCCCCACGCTTT	CGCTGGATTCCACGAAGTTT	45.0	50.0	58.0	58.8	1087-1090 bp	1.0	74.2
ccsA_p2	AGTGAAATCCCCACGCTTTT	CGCTGGATTCCACGAAGTTT	45.0	50.0	58.0	58.8	1086-1089 bp	1.0	74.2
ccsA_p3	AAGTGAAATCCCCACGCTTT	CGCTGGATTCCACGAAGTT	45.0	52.6	58.0	58.2	1087-1090 bp	1.0	71.6
ccsA_p4	AGTGAAATCCCCACGCTTTT	CGCTGGATTCCACGAAGTT	45.0	52.6	58.0	58.2	1086-1089 bp	1.0	71.6
ccsA_p5	AGTGAAATCCCCACGCTTTT	GCTGGATTCCACGAAGTTTTC	45.0	47.6	58.0	57.8	1085-1088 bp	1.0	71.3