Hotspot

rps15-ycf1

SSC · 124174-126213 · 2040 bp

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Pi 0.0174

MarkerSeek score 48.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 2040
variable_sites: 71
pi: 0.0174
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGGAGGCCTCTCTGAGATAA	TTTTGGTTTGCCACGAACAC	55.0	45.0	58.0	58.0	2876-2881 bp	1.0	82.5
rps15-ycf1_p2	CGGAGGCCTCTCTGAGATAA	CTTCCTTTTGGTTTGCCACG	55.0	50.0	58.0	58.1	2881-2886 bp	1.0	81.3
rps15-ycf1_p3	TAAGACAGCATCCTTTGGCG	TTTTGGTTTGCCACGAACAC	50.0	45.0	58.3	58.0	2912-2917 bp	1.0	80.5
rps15-ycf1_p4	CGGAGGCCTCTCTGAGATAA	GTTTGCCACGAACACAACTT	55.0	45.0	58.0	57.7	2871-2876 bp	1.0	80.3
rps15-ycf1_p5	CGGAGGCCTCTCTGAGATAA	GGGGAATGGAAACCGAACTT	55.0	50.0	58.0	58.1	2901-2903 bp	0.6	65.2