Hotspot

ccsA-ndhD

SSC · 115891-116163 · 273 bp

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Pi 0.0454

MarkerSeek score 69.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 273
variable_sites: 21
pi: 0.0454
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTGCAATTGTGGCTTCGATG	GCGCGAATTGTTTCTTTCGA	50.0	45.0	58.1	58.1	513 bp	1.0	82.2
ccsA-ndhD_p2	GCTGGGCGAGATTCAATACA	GCGCGAATTGTTTCTTTCGA	50.0	45.0	58.0	58.1	815 bp	1.0	81.5
ccsA-ndhD_p3	GCTGGGCGAGATTCAATACA	TCAACGGGGCCATTTTACAA	50.0	45.0	58.0	58.0	1209 bp	1.0	81.3
ccsA-ndhD_p4	CTGCAATTGTGGCTTCGATG	TCAACGGGGCCATTTTACAA	50.0	45.0	58.1	58.0	907 bp	1.0	81.2
ccsA-ndhD_p5	TCAGGAGCAGTATGGGCTAA	TCAACGGGGCCATTTTACAA	50.0	45.0	57.8	58.0	1046 bp	1.0	80.4