Hotspot

rpl32-trnL-UAG

SSC · 113795-114725 · 931 bp

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Pi 0.023

MarkerSeek score 70.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 931
variable_sites: 40
pi: 0.023
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAAGGATATTGGGCAGCG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1037-1055 bp	1.0	84.3
rpl32-trnL-UAG_p2	AGAAAGGATATTGGGCAGCG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1038-1056 bp	1.0	84.3
rpl32-trnL-UAG_p3	AAAGGATATTGGGCAGCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1035-1053 bp	1.0	83.4
rpl32-trnL-UAG_p4	AAAGGATATTGGGCAGCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1036-1054 bp	1.0	83.4
rpl32-trnL-UAG_p5	AGAAAGGATATTGGGCAGCG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1047-1065 bp	1.0	83.3