Hotspot

psaI-ycf4

LSC · 60095-60558 · 464 bp

Back to Pellionia

Pi 0.0112

MarkerSeek score 57.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 10
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TCCGGCAATTGCTATGGTTT	TCGAGACCCCGCTATAAGTT	45.0	50.0	58.2	57.9	573 bp	1.0	79.7
psaI-ycf4_p2	TCCGGCAATTGCTATGGTTT	TTCGAGACCCCGCTATAAGT	45.0	50.0	58.2	57.9	574 bp	1.0	79.7
psaI-ycf4_p3	TTCCGGCAATTGCTATGGTT	TCGAGACCCCGCTATAAGTT	45.0	50.0	58.2	57.9	574 bp	1.0	79.7
psaI-ycf4_p4	TTCCGGCAATTGCTATGGTT	TTCGAGACCCCGCTATAAGT	45.0	50.0	58.2	57.9	575 bp	1.0	79.7
psaI-ycf4_p5	TTTCCGGCAATTGCTATGGT	TCGAGACCCCGCTATAAGTT	45.0	50.0	58.2	57.9	575 bp	1.0	79.7