Hotspot

rps4-trnT-UGU

LSC · 46619-47113 · 495 bp

Back to Pellionia

Pi 0.0133

MarkerSeek score 61.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 12
pi: 0.0133
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCCAGACGACGTATTTTT	GCATTTGTAATGCGGTGGTC	50.0	50.0	57.9	58.1	510-589 bp	1.0	84.2
rps4-trnT-UGU_p2	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	528-607 bp	1.0	83.8
rps4-trnT-UGU_p3	GAAACGAGGCCCTCTGTAAC	GCATTTGTAATGCGGTGGTC	55.0	50.0	58.3	58.1	488-567 bp	1.0	82.9
rps4-trnT-UGU_p4	CCGAGGCGTAATTGGTTTCT	GCATTTGTAATGCGGTGGTC	50.0	50.0	58.3	58.1	577-656 bp	1.0	82.7
rps4-trnT-UGU_p5	CCGAGGCGTAATTGGTTTCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	595-674 bp	1.0	82.3