Hotspot

trnG-GCC-trnfM-CAU

LSC · 36820-36985 · 166 bp

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Pi 0.0251
MarkerSeek score 63.90
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
166
variable_sites
8
pi
0.0251
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 229-233 bp 1.0 83.7
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 230-234 bp 1.0 83.7
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 265-269 bp 1.0 83.2
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 266-270 bp 1.0 83.2
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC GCAGTTTGGTAGCTTGCAAG 50.0 50.0 58.1 57.9 258-262 bp 1.0 83.1