Hotspot

atpA-atpF

LSC · 11850-11912 · 63 bp

Back to Pellionia

Pi 0.0619

MarkerSeek score 69.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 8
pi: 0.0619
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TTTCATCGGCTCGAATGGTT	TGGAACAATGAAAGAAATAACGGA	45.0	33.3	57.9	57.1	115-116 bp	1.0	46.3
atpA-atpF_p2	TCATCGGCTCGAATGGTTAC	TGGAACAATGAAAGAAATAACGGA	50.0	33.3	57.8	57.1	113-114 bp	1.0	46.3
atpA-atpF_p3	TCATCGGCTCGAATGGTTAC	TGGAACAATGAAAGAAATAACGG	50.0	34.8	57.8	55.8	113-114 bp	1.0	46.3
atpA-atpF_p4	ATTTCATCGGCTCGAATGGT	TGGAACAATGAAAGAAATAACGGA	45.0	33.3	57.7	57.1	116-117 bp	1.0	46.2
atpA-atpF_p5	TTTCATCGGCTCGAATGGTT	TGGAACAATGAAAGAAATAACGG	45.0	34.8	57.9	55.8	115-116 bp	1.0	46.2