Hotspot

rps16-trnQ-UUG

LSC · 6210-6975 · 766 bp

Back to Pellionia

Pi 0.016

MarkerSeek score 60.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 24
pi: 0.016
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	838-861 bp	1.0	83.8
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	853-876 bp	1.0	81.8
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	852-875 bp	1.0	81.8
rps16-trnQ-UUG_p4	TGGGTGTAAGAATCCACAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	872-895 bp	1.0	80.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	859-882 bp	1.0	80.0