Hotspot

matK-trnK-UUU

LSC · 3443-4204 · 762 bp

Back to Pellionia

Pi 0.0129

MarkerSeek score 57.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 18
pi: 0.0129
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AAGTGGGTATAGGAGGTCAT	AACGGTAGAGTACTCGGCTT	45.0	50.0	54.6	58.2	851-872 bp	1.0	53.0
matK-trnK-UUU_p2	AAGTGGGTATAGGAGGTCAT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	54.6	58.2	850-871 bp	1.0	53.0
matK-trnK-UUU_p3	AAGTGGGTATAGGAGGTCAT	GGGTTGCTAACTCAACGGTA	45.0	50.0	54.6	57.5	864-885 bp	1.0	50.6
matK-trnK-UUU_p4	AAGTGGGTATAGGAGGTCAT	TTCGGGTTGCTAACTCAACG	45.0	50.0	54.6	58.5	867-888 bp	1.0	50.3
matK-trnK-UUU_p5	AAGTGGGTATAGGAGGTCAT	CAACGGTAGAGTACTCGGCT	45.0	55.0	54.6	58.9	852-873 bp	1.0	47.1