Hotspot

rps2

Genome · 16400-17110 · 711 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
Genome
length_bp
711
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 TCCCATGCATATGCGTATGT CACCACCACTACCATTACCG 45.0 55.0 57.1 58.0 854 bp 1.0 73.0
rps2_p2 TCCCATGCATATGCGTATGT ATCACTCACCACCACTACCA 45.0 50.0 57.1 58.0 860 bp 1.0 72.9
rps2_p3 TCCCATGCATATGCGTATGT TCACTCACCACCACTACCAT 45.0 50.0 57.1 58.0 859 bp 1.0 72.9
rps2_p4 CCCATGCATATGCGTATGTG CACCACCACTACCATTACCG 50.0 55.0 57.1 58.0 853 bp 1.0 72.6
rps2_p5 CCCATGCATATGCGTATGTG TCACTCACCACCACTACCAT 50.0 50.0 57.1 58.0 858 bp 1.0 72.6