Hotspot

rps18

Genome · 65020-65247 · 228 bp

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Pi 0.00439
MarkerSeek score 55.00
Primer available yes
Label rps18

Feature Metadata

label_name
rps18
feature_type
gene
region
Genome
length_bp
228
variable_sites
1
pi
0.00439
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18_p1 TGGGTGCACTATCCACAAAG CCTCCCCCGGTTAATTTCTG 50.0 55.0 57.8 57.9 355 bp 1.0 78.2
rps18_p2 TGGGTGCACTATCCACAAAG CTCCCCCGGTTAATTTCTGG 50.0 55.0 57.8 57.9 354 bp 1.0 78.2
rps18_p3 TACTGTGGTGGGTGCACTAT CCTCCCCCGGTTAATTTCTG 50.0 55.0 58.3 57.9 363 bp 1.0 77.0
rps18_p4 TACTGTGGTGGGTGCACTAT CTCCCCCGGTTAATTTCTGG 50.0 55.0 58.3 57.9 362 bp 1.0 77.0
rps18_p5 TTTTGTCGCTACTGTGGTGG CTCCCCCGGTTAATTTCTGG 50.0 55.0 58.4 57.9 371 bp 1.0 76.5