Hotspot

psbZ

Genome · 41148-41336 · 189 bp

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Pi 0.00529
MarkerSeek score 55.50
Primer available yes
Label psbZ

Feature Metadata

label_name
psbZ
feature_type
gene
region
Genome
length_bp
189
variable_sites
1
pi
0.00529
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ_p1 CTCGAACAGTTTTACGGGGA TCGAAACACTTGCTCTAAATGC 50.0 40.9 57.6 57.6 394 bp 1.0 57.4
psbZ_p2 TCTCGAACAGTTTTACGGGG TCGAAACACTTGCTCTAAATGC 50.0 40.9 57.6 57.6 395 bp 1.0 57.4
psbZ_p3 CTCGAACAGTTTTACGGGGA CGAAACACTTGCTCTAAATGCT 50.0 40.9 57.6 57.6 393 bp 1.0 57.4
psbZ_p4 TCTCGAACAGTTTTACGGGG CGAAACACTTGCTCTAAATGCT 50.0 40.9 57.6 57.6 394 bp 1.0 57.4
psbZ_p5 CTCGAACAGTTTTACGGGGA GGATGTAAACCAAGGAGGATGA 50.0 45.5 57.6 57.5 355 bp 1.0 56.6