Hotspot

psbB

Genome · 68514-70031 · 1518 bp

Pi 0.000659

MarkerSeek score 59.10

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: Genome
length_bp: 1518
variable_sites: 1
pi: 0.000659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	GCGGTTTCTTTCGATGCAAA	AGAAGAAACGATGATGATGCCT	45.0	40.9	58.0	57.9	1667 bp	1.0	62.5
psbB_p2	TAGCGGTTTCTTTCGATGCA	AGAAGAAACGATGATGATGCCT	45.0	40.9	57.9	57.9	1669 bp	1.0	62.2
psbB_p3	GTAGCGGTTTCTTTCGATGC	AGAAGAAACGATGATGATGCCT	50.0	40.9	57.6	57.9	1670 bp	1.0	59.4
psbB_p4	GCGGTTTCTTTCGATGCAAA	AGAAGAAACGATGATGATGCC	45.0	42.9	58.0	56.4	1667 bp	1.0	58.9
psbB_p5	GCGGTTTCTTTCGATGCAAA	GAAGAAACGATGATGATGCCT	45.0	42.9	58.0	56.4	1666 bp	1.0	58.9