Pi
0.0217
MarkerSeek score
67.20
Primer available
yes
Label
ndhF-rpl21
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhF-rpl21_p1 | AAAAAGTGATCCCGATCCCG | CCAATCGATTCGGTGTCAGT | 50.0 | 50.0 | 58.0 | 58.0 | 608 bp | 1.0 | 80.8 |
| ndhF-rpl21_p2 | GAAACAGTGGTACGAGCCAA | CCAATCGATTCGGTGTCAGT | 50.0 | 50.0 | 58.1 | 58.0 | 567 bp | 1.0 | 80.2 |
| ndhF-rpl21_p3 | AACGGAAACAGTGGTACGAG | CCAATCGATTCGGTGTCAGT | 50.0 | 50.0 | 57.9 | 58.0 | 571 bp | 1.0 | 80.0 |
| ndhF-rpl21_p4 | AAAAAGTGATCCCGATCCCG | TCGTGTTTCGTTCCAATCGA | 50.0 | 45.0 | 58.0 | 57.9 | 620 bp | 1.0 | 79.8 |
| ndhF-rpl21_p5 | GATCCCGGTAGAATGGAAGC | CCAATCGATTCGGTGTCAGT | 55.0 | 50.0 | 57.8 | 58.0 | 595 bp | 1.0 | 79.8 |