Hotspot

trnR-CCG-accD

Genome · 57420-57627 · 208 bp

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Pi 0
MarkerSeek score 52.30
Primer available yes
Label trnR-CCG-accD

Feature Metadata

label_name
trnR-CCG-accD
feature_type
igs
region
Genome
length_bp
208
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-CCG-accD_p1 CCGAATCATCAGGTCAAGGG CCTTTCGTCGCTTTTTCGAT 55.0 45.0 58.0 57.1 327-328 bp 1.0 72.7
trnR-CCG-accD_p2 TGGTAGCTCAGCGGATTAGA CCTTTCGTCGCTTTTTCGAT 50.0 45.0 57.9 57.1 357-358 bp 1.0 72.5
trnR-CCG-accD_p3 CCTTTTCGGAGACTTCCCAG CCTTTCGTCGCTTTTTCGAT 55.0 45.0 57.9 57.1 389-390 bp 1.0 72.2
trnR-CCG-accD_p4 TCCTTTTCGGAGACTTCCCA CCTTTCGTCGCTTTTTCGAT 50.0 45.0 58.3 57.1 390-391 bp 1.0 70.8
trnR-CCG-accD_p5 CCGAATCATCAGGTCAAGGG TAGCCTTTCGTCGCTTTTTC 55.0 45.0 58.0 56.8 330-331 bp 1.0 69.7