Hotspot

trnD-GUC-trnY-GUA

Genome · 36710-36866 · 157 bp

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Pi 0.00637
MarkerSeek score 56.20
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
Genome
length_bp
157
variable_sites
1
pi
0.00637
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TTGACAGGGCGGTACTCTAA ACTGTAAATCCGCTGGCAAT 50.0 45.0 58.1 57.9 247 bp 1.0 78.7
trnD-GUC-trnY-GUA_p2 TTGACAGGGCGGTACTCTAA CTCGAGTGGTTAATGGGGAC 50.0 55.0 58.1 57.7 269 bp 1.0 77.2
trnD-GUC-trnY-GUA_p3 TTGACAGGGCGGTACTCTAA ATGCTCGAGTGGTTAATGGG 50.0 50.0 58.1 57.7 272 bp 1.0 77.1
trnD-GUC-trnY-GUA_p4 TTGACAGGGCGGTACTCTAA CTGTAAATCCGCTGGCAATG 50.0 50.0 58.1 57.5 246 bp 1.0 75.9
trnD-GUC-trnY-GUA_p5 CAACTTCCGTCTTGACAGGG ACTGTAAATCCGCTGGCAAT 55.0 45.0 58.5 57.9 258 bp 1.0 75.4