Hotspot

rpl16

LSC · 82063-83711 · 1649 bp

Back to Pelatantheria

Pi 0.0245

MarkerSeek score 61.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1649
variable_sites: 40
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACTCCTTCTTCTTTTACTTTTGT	TCAAAGAAAAACGAACAATTAGA	30.4	26.1	54.1	53.2	1759-1862 bp	1.0	42.4
rpl16_p2	ACTCCTTCTTCTTTTACTTTTGT	TCTTAATCAAAGAAAAACGAACA	30.4	26.1	54.1	53.2	1765-1868 bp	1.0	42.4
rpl16_p3	ACTCCTTCTTCTTTTACTTTTGT	TCAAAGAAAAACGAACAATTAGAA	30.4	25.0	54.1	54.0	1759-1862 bp	1.0	42.4
rpl16_p4	ACTCCTTCTTCTTTTACTTTTGT	TCTTAATCAAAGAAAAACGAACAA	30.4	25.0	54.1	54.0	1765-1868 bp	1.0	42.4
rpl16_p5	ACTCCTTCTTCTTTTACTTTTGTT	TCAAAGAAAAACGAACAATTAGA	29.2	26.1	54.8	53.2	1759-1862 bp	1.0	42.4