Hotspot

trnL-CAA-ndhB

IRb · 95606-96351 · 746 bp

Back to Pelatantheria

Pi 0.0483

MarkerSeek score 71.30

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 746
variable_sites: 29
pi: 0.0483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	CTCAGGTCGATGGATCTCCT	CCCTTTTCGGACTCCTGATG	55.0	55.0	58.0	58.0	1430 bp	0.5	59.7
trnL-CAA-ndhB_p2	GTCTCGCGTGTCTACCATTT	CCCTTTTCGGACTCCTGATG	50.0	55.0	58.0	58.0	809 bp	0.5	59.7
trnL-CAA-ndhB_p3	CTCGCGTGTCTACCATTTCA	CCCTTTTCGGACTCCTGATG	50.0	55.0	58.0	58.0	807 bp	0.5	59.7
trnL-CAA-ndhB_p4	TCAGGTCGATGGATCTCCTC	CCCTTTTCGGACTCCTGATG	55.0	55.0	58.0	58.0	1429 bp	0.5	59.7
trnL-CAA-ndhB_p5	TCTCAGGTCGATGGATCTCC	CCCTTTTCGGACTCCTGATG	55.0	55.0	58.0	58.0	1431 bp	0.5	59.7