Hotspot

ndhC-trnV-UAC

LSC · 49968-50604 · 637 bp

Back to Pelatantheria

Pi 0.064
MarkerSeek score 78.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
637
variable_sites
40
pi
0.064
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCAGAAAATCTCATATCATATTCGTGA GTTCGAGTCCGTATAGCCCT 33.3 55.0 57.6 58.7 696-757 bp 1.0 45.7
ndhC-trnV-UAC_p2 CCAGAAAATCTCATATCATATTCGTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 57.6 57.8 708-769 bp 1.0 45.6
ndhC-trnV-UAC_p3 CCAGAAAATCTCATATCATATTCGTG AGAAGGTCTACGGTTCGAGT 34.6 50.0 56.4 57.8 708-769 bp 1.0 45.6
ndhC-trnV-UAC_p4 CCAGAAAATCTCATATCATATTCGTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 57.6 57.5 707-768 bp 1.0 45.6
ndhC-trnV-UAC_p5 CCAGAAAATCTCATATCATATTCGT AGAAGGTCTACGGTTCGAGT 32.0 50.0 55.1 57.8 708-769 bp 1.0 45.6