Hotspot

rpoA-petD

IRa · 172072-172992 · 921 bp

Back to Pelargonium

Pi 0.256

MarkerSeek score 78.90

Primer available yes

Label rpoA-petD

Feature Metadata

label_name: rpoA-petD
feature_type: igs
region: IRa
length_bp: 921
variable_sites: 561
pi: 0.256
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-petD_p1	GTACCACCGAGACGACTAGA	GGTTGGGTATTGGAGCAACA	55.0	50.0	58.0	58.1	1320-1443 bp	0.615385	75.8
rpoA-petD_p2	GTACCACCGAGACGACTAGA	TGGTTGGGTATTGGAGCAAC	55.0	50.0	58.0	58.1	1321-1444 bp	0.615385	75.8
rpoA-petD_p3	AAGACTCGGAAGACTGTCCA	GGTTGGGTATTGGAGCAACA	50.0	50.0	58.0	58.1	1007-1130 bp	0.461538	69.3
rpoA-petD_p4	AAGACTCGGAAGACTGTCCA	TGGTTGGGTATTGGAGCAAC	50.0	50.0	58.0	58.1	1008-1131 bp	0.461538	69.3
rpoA-petD_p5	AAGACTCGGAAGACTGTCCA	GTTTCAGTACCTGCGGGATT	50.0	50.0	58.0	57.9	1128-1251 bp	0.461538	68.3