Hotspot

petD-rpoA

IRb · 90894-91814 · 921 bp

Back to Pelargonium

Pi 0.255

MarkerSeek score 78.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: IRb
length_bp: 921
variable_sites: 555
pi: 0.255
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	GTACCACCGAGACGACTAGA	50.0	55.0	58.1	58.0	1320-1443 bp	0.615385	75.8
petD-rpoA_p2	CTCGAGCCGGATGATGAAAA	GTACCACCGAGACGACTAGA	50.0	55.0	58.1	58.0	1812-1935 bp	0.615385	74.3
petD-rpoA_p3	GGTTGGGTATTGGAGCAACA	AAGACTCGGAAGACTGTCCA	50.0	50.0	58.1	58.0	1007-1130 bp	0.461538	69.3
petD-rpoA_p4	TGGTTGGGTATTGGAGCAAC	AAGACTCGGAAGACTGTCCA	50.0	50.0	58.1	58.0	1008-1131 bp	0.461538	69.3
petD-rpoA_p5	CTCGAGCCGGATGATGAAAA	AAGACTCGGAAGACTGTCCA	50.0	50.0	58.1	58.0	1499-1622 bp	0.461538	66.8