Hotspot

petA-psbJ

LSC · 75418-76524 · 1107 bp

Back to Pelargonium

Pi 0.045

MarkerSeek score 82.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1107
variable_sites: 161
pi: 0.045
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1838-1889 bp	1.0	90.4
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1837-1888 bp	1.0	90.4
petA-psbJ_p3	CTGACGGGAGCAAGAGTAAC	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1883-1934 bp	1.0	90.3
petA-psbJ_p4	CTGACGGGAGCAAGAGTAAC	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	2096-2147 bp	1.0	90.0
petA-psbJ_p5	CTGACGGGAGCAAGAGTAAC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	2095-2146 bp	1.0	90.0