Hotspot

trnE-UUC-trnD-GUC

LSC · 37299-40560 · 3262 bp

Back to Pelargonium

Pi 0.323

MarkerSeek score 70.90

Primer available yes

Label trnE-UUC-trnD-GUC

Feature Metadata

label_name: trnE-UUC-trnD-GUC
feature_type: igs
region: LSC
length_bp: 3262
variable_sites: 1804
pi: 0.323
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnD-GUC_p1	ATGCGTTGTGCCATTTCATG	ATCATAATGTTTCCGCCCCC	45.0	50.0	58.0	58.0	1855 bp	0.076923	43.0
trnE-UUC-trnD-GUC_p2	ATGCGTTGTGCCATTTCATG	GGCCATTTCCGAAATTGTGG	45.0	50.0	58.0	58.0	1881 bp	0.076923	43.0
trnE-UUC-trnD-GUC_p3	ATGCGTTGTGCCATTTCATG	TCAAGGAAAAAGCGAGCACT	45.0	45.0	58.0	58.0	2295 bp	0.076923	42.8
trnE-UUC-trnD-GUC_p4	ATGCGTTGTGCCATTTCATG	GTCAAGGAAAAAGCGAGCAC	45.0	50.0	58.0	58.0	2296 bp	0.076923	42.8
trnE-UUC-trnD-GUC_p5	ATGCGTTGTGCCATTTCATG	CAAGGAAAAAGCGAGCACTG	45.0	50.0	58.0	58.0	2294 bp	0.076923	42.7