primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGGTCTTACAAACCCTACCG	GGATCTTCGAAAGGTGGGTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026018	12287	12894	892-905	0.846154	85.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCAACCCCTAATCGAATGGG	GGATCTTCGAAAGGTGGGTC	20	20	55.0	55.0	58.0	58.0	12.2	0.0	0.044332	12287	12894	1240-1255	0.846154	83.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	GGTGGGGAATTTCTTGGGTT	GGATCTTCGAAAGGTGGGTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.049764	12287	12894	1508-1520	0.615385	70.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGACGGTATGGACGAATCTC	GGATCTTCGAAAGGTGGGTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.059382	12287	12894	875-887	0.538462	68.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CTTACAAACCCTACCGACGG	GGATCTTCGAAAGGTGGGTC	20	20	55.0	55.0	58.0	58.0	13.6	0.0	0.026018	12287	12894	889-900	0.307692	56.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	CACTTTTACCACTAAACTATACCCG	20	25	50.0	40.0	58.0	57.5	0.0	0.0	5.534463	14874	15768	914-994	1.000000	52.0
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	CACTTTTACCACTAAACTATACCCG	20	25	50.0	40.0	58.0	57.5	0.0	0.0	5.535851	14874	15768	913-993	1.000000	52.0
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	ACTTTTACCACTAAACTATACCCG	20	24	50.0	37.5	58.0	56.2	0.0	0.0	5.792359	14874	15768	913-993	1.000000	52.0
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	ACTTTTACCACTAAACTATACCCG	20	24	50.0	37.5	58.0	56.2	0.0	0.0	5.793747	14874	15768	912-992	1.000000	52.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGCAATCCGACGCTTTAGTC	CACTTTTACCACTAAACTATACCCG	20	25	50.0	40.0	58.4	57.5	0.0	0.0	5.888303	14874	15768	916-996	1.000000	52.0
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	ATGGAGGGTCATCATTGA	20	18	45.0	44.4	58.1	52.2	1.4	0.0	7.875794	20661	21598	985-1242	1.000000	52.0
atpH-atpI_p2	atpH-atpI	2	GCAGAAATAACAGAAGCGGC	ATGGAGGGTCATCATTGA	20	18	50.0	44.4	57.8	52.2	0.0	0.0	7.951963	20661	21598	993-1250	1.000000	52.0
atpH-atpI_p3	atpH-atpI	3	GGCCAACCCAGCAGAAATAA	ATGGAGGGTCATCATTGA	20	18	50.0	44.4	58.4	52.2	0.0	0.0	8.216047	20661	21598	1003-1260	1.000000	52.0
atpH-atpI_p4	atpH-atpI	4	AGAAGCGGCAGAAATCAGTG	ATGGAGGGTCATCATTGA	20	18	50.0	44.4	58.6	52.2	1.4	0.0	8.325107	20661	21598	982-1239	1.000000	52.0
atpH-atpI_p5	atpH-atpI	5	CAGAAGCGGCAGAAATCAGT	ATGGAGGGTCATCATTGA	20	18	50.0	44.4	58.6	52.2	1.4	0.0	8.325107	20661	21598	983-1240	1.000000	52.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCTTCATTTCTATCGCCAAGC	AGGATTTGCAGTCCCCCG	21	18	47.6	61.1	57.4	59.3	0.0	14.6	4.938621	33790	34894	935-1190	1.000000	52.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GGGATTGTAGCCATTCCTTCA	AGGATTTGCAGTCCCCCG	21	18	47.6	61.1	57.7	59.3	0.0	14.6	4.619907	33790	34894	950-1205	0.923077	52.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AGGGATTGTAGCCATTCCTTC	AGGATTTGCAGTCCCCCG	21	18	47.6	61.1	57.4	59.3	0.0	14.6	4.907505	33790	34894	951-1206	0.923077	49.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GGGATTGTAGCCATTCCTTCA	CTTACCGCTCGGCCATGT	21	18	47.6	61.1	57.7	59.8	0.0	8.4	5.110231	33790	34894	931-1186	0.923077	48.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GGGATTGTAGCCATTCCTTCA	TTACCGCTCGGCCATGTC	21	18	47.6	61.1	57.7	59.8	0.0	8.4	5.111268	33790	34894	930-1185	0.923077	48.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	20	20	40.0	50.0	54.5	58.5	0.0	5.4	3.996878	35660	36765	550-1271	0.846154	53.9
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ATGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	21	20	38.1	50.0	55.1	58.5	0.0	5.4	4.379605	35660	36765	551-1272	0.846154	50.8
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TGCGAGAATATTGACTTCCAT	TTCAATTGGTCAGAGCACCG	21	20	38.1	50.0	55.1	58.5	0.0	5.4	4.379605	35660	36765	550-1271	0.846154	50.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TGCGAGAATATTGACTTCCA	GTTCAATTGGTCAGAGCACC	20	20	40.0	50.0	54.5	57.0	0.0	5.4	4.517032	35660	36765	551-1272	0.846154	49.7
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AATGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	22	20	36.4	50.0	55.9	58.5	0.0	5.4	4.600216	35660	36765	552-1273	0.846154	49.0
trnE-UUC-trnD-GUC_p1	trnE-UUC-trnD-GUC	1	ATGCGTTGTGCCATTTCATG	ATCATAATGTTTCCGCCCCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.005575	38519	38638	1855-1855	0.076923	43.0
trnE-UUC-trnD-GUC_p2	trnE-UUC-trnD-GUC	2	ATGCGTTGTGCCATTTCATG	GGCCATTTCCGAAATTGTGG	20	20	45.0	50.0	58.0	58.0	0.0	3.5	0.009703	38519	38638	1881-1881	0.076923	43.0
trnE-UUC-trnD-GUC_p3	trnE-UUC-trnD-GUC	3	ATGCGTTGTGCCATTTCATG	TCAAGGAAAAAGCGAGCACT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.033633	38519	38638	2295-2295	0.076923	42.8
trnE-UUC-trnD-GUC_p4	trnE-UUC-trnD-GUC	4	ATGCGTTGTGCCATTTCATG	GTCAAGGAAAAAGCGAGCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.038892	38519	38638	2296-2296	0.076923	42.8
trnE-UUC-trnD-GUC_p5	trnE-UUC-trnD-GUC	5	ATGCGTTGTGCCATTTCATG	CAAGGAAAAAGCGAGCACTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.043056	38519	38638	2294-2294	0.076923	42.7
trnD-GUC-trnG-GCC_p1	trnD-GUC-trnG-GCC	1	ATGGATCTTGTGGCCGATTC	ACTTCACCTTCAACAAGCGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.065515	42205	42876	1729-2440	0.153846	56.0
trnD-GUC-trnG-GCC_p2	trnD-GUC-trnG-GCC	2	AACCGATTCTGCGAGACTTC	ACTTCACCTTCAACAAGCGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.040718	42205	42876	1331-1331	0.076923	42.8
trnD-GUC-trnG-GCC_p3	trnD-GUC-trnG-GCC	3	AACCGATTCTGCGAGACTTC	TTCTCCTGGGCTTGTAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041194	42205	42876	1179-1179	0.076923	42.7
trnD-GUC-trnG-GCC_p4	trnD-GUC-trnG-GCC	4	AACCGATTCTGCGAGACTTC	TTTCTCCTGGGCTTGTAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041194	42205	42876	1180-1180	0.076923	42.7
trnD-GUC-trnG-GCC_p5	trnD-GUC-trnG-GCC	5	AACCGATTCTGCGAGACTTC	TGAGGTTGCATTAATCGGGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.053665	42205	42876	1389-1389	0.076923	42.6
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043011	75418	76524	1838-1889	1.000000	90.4
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043011	75418	76524	1837-1888	1.000000	90.4
petA-psbJ_p3	petA-psbJ	3	CTGACGGGAGCAAGAGTAAC	ACACAATCAAACCCGAACGA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.046034	75418	76524	1883-1934	1.000000	90.3
petA-psbJ_p4	petA-psbJ	4	CTGACGGGAGCAAGAGTAAC	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.006955	75418	76524	2096-2147	1.000000	90.0
petA-psbJ_p5	petA-psbJ	5	CTGACGGGAGCAAGAGTAAC	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.006955	75418	76524	2095-2146	1.000000	90.0
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	AAGCGACGAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.3	24.9	0.0	0.461704	77334	78203	926-1039	1.000000	88.3
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GCGACGAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.3	24.9	0.0	0.466384	77334	78203	924-1037	1.000000	88.3
psbE-petL_p3	psbE-petL	3	GTTCTCCTGTGCTTCCAGAC	AAGCGACGAGTAGAAAACCG	20	20	55.0	50.0	58.2	58.3	0.9	0.0	0.503292	77334	78203	921-1034	1.000000	88.0
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	GCGACGAGTAGAAAACCGAA	20	20	55.0	50.0	58.2	58.3	0.9	0.0	0.507972	77334	78203	919-1032	1.000000	87.9
psbE-petL_p5	psbE-petL	5	TCTCCTGTGCTTCCAGACAT	AAGCGACGAGTAGAAAACCG	20	20	50.0	50.0	58.4	58.3	0.9	0.0	0.656039	77334	78203	919-1032	1.000000	86.8
petD-rpoA_p1	petD-rpoA	1	GGTTGGGTATTGGAGCAACA	GTACCACCGAGACGACTAGA	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.097864	90894	91814	1320-1443	0.615385	75.8
petD-rpoA_p2	petD-rpoA	2	CTCGAGCCGGATGATGAAAA	GTACCACCGAGACGACTAGA	20	20	50.0	55.0	58.1	58.0	5.0	0.0	0.075762	90894	91814	1812-1935	0.615385	74.3
petD-rpoA_p3	petD-rpoA	3	GGTTGGGTATTGGAGCAACA	AAGACTCGGAAGACTGTCCA	20	20	50.0	50.0	58.1	58.0	0.0	3.8	0.085330	90894	91814	1007-1130	0.461538	69.3
petD-rpoA_p4	petD-rpoA	4	TGGTTGGGTATTGGAGCAAC	AAGACTCGGAAGACTGTCCA	20	20	50.0	50.0	58.1	58.0	0.0	3.8	0.085330	90894	91814	1008-1131	0.461538	69.3
petD-rpoA_p5	petD-rpoA	5	CTCGAGCCGGATGATGAAAA	AAGACTCGGAAGACTGTCCA	20	20	50.0	50.0	58.1	58.0	5.0	3.8	0.063227	90894	91814	1499-1622	0.461538	66.8
rpoA_p1	rpoA	1	TCTTGGAAGCTTTCGTCGAG	TTAGAAGTGGCGTCCGATTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070575	91815	92699	1884-2072	0.615385	75.1
rpoA_p2	rpoA	2	CCGATTCTTCCGTTCCCATT	TTAGAAGTGGCGTCCGATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042969	91815	92699	2229-2313	0.384615	64.8
rpoA_p3	rpoA	3	CCGATTCTTCCGTTCCCATT	AAGGGATGCAGCATTACGAG	20	20	50.0	50.0	58.0	58.0	0.0	9.9	0.079345	91815	92699	2255-2339	0.384615	64.5
rpoA_p4	rpoA	4	CCGATTCTTCCGTTCCCATT	GAAGGGATGCAGCATTACGA	20	20	50.0	50.0	58.0	58.0	0.0	9.9	0.084828	91815	92699	2256-2340	0.384615	64.4
rpoA_p5	rpoA	5	ACAAGCAAAGCCCCTTCTAC	TTAGAAGTGGCGTCCGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.093525	91815	92699	1908-2096	0.307692	58.5
ndhF_p1	ndhF	1	TGAAGAACCCCAGCTTTACC	GAGCTACTGAGGAACAACGG	20	20	50.0	55.0	57.4	58.0	0.0	0.0	0.581741	147174	149408	2925-2928	0.615385	60.7
ndhF_p2	ndhF	2	TGAAGAACCCCAGCTTTACC	CCCGGAACTTCTTCGTAGTG	20	20	50.0	55.0	57.4	58.0	0.0	0.7	0.587240	147174	149408	2830-2833	0.615385	60.7
ndhF_p3	ndhF	3	TGAAGAACCCCAGCTTTACC	CCGGAACTTCTTCGTAGTGG	20	20	50.0	55.0	57.4	58.0	0.0	0.0	0.587240	147174	149408	2829-2832	0.615385	60.7
ndhF_p4	ndhF	4	TGAAGAACCCCAGCTTTACC	ACTCCCGGAACTTCTTCGTA	20	20	50.0	50.0	57.4	58.1	0.0	0.7	0.664269	147174	149408	2833-2836	0.615385	60.1
ndhF_p5	ndhF	5	TGAAGAACCCCAGCTTTACC	TCCCGGAACTTCTTCGTAGT	20	20	50.0	50.0	57.4	58.1	0.0	0.7	0.664269	147174	149408	2831-2834	0.615385	60.1
rpoA_p1	rpoA	1	TTAGAAGTGGCGTCCGATTG	TCTTGGAAGCTTTCGTCGAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070575	171187	172071	1884-2072	0.615385	75.1
rpoA_p2	rpoA	2	TTAGAAGTGGCGTCCGATTG	CCGATTCTTCCGTTCCCATT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042969	171187	172071	2229-2313	0.384615	64.9
rpoA_p3	rpoA	3	AAGGGATGCAGCATTACGAG	CCGATTCTTCCGTTCCCATT	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.079345	171187	172071	2255-2339	0.384615	64.6
rpoA_p4	rpoA	4	GAAGGGATGCAGCATTACGA	CCGATTCTTCCGTTCCCATT	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.084828	171187	172071	2256-2340	0.384615	64.5
rpoA_p5	rpoA	5	TTAGAAGTGGCGTCCGATTG	ACAAGCAAAGCCCCTTCTAC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.093525	171187	172071	1908-2096	0.307692	58.5
rpoA-petD_p1	rpoA-petD	1	GTACCACCGAGACGACTAGA	GGTTGGGTATTGGAGCAACA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.097864	172072	172992	1320-1443	0.615385	75.8
rpoA-petD_p2	rpoA-petD	2	GTACCACCGAGACGACTAGA	TGGTTGGGTATTGGAGCAAC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.097864	172072	172992	1321-1444	0.615385	75.8
rpoA-petD_p3	rpoA-petD	3	AAGACTCGGAAGACTGTCCA	GGTTGGGTATTGGAGCAACA	20	20	50.0	50.0	58.0	58.1	3.8	0.0	0.085330	172072	172992	1007-1130	0.461538	69.3
rpoA-petD_p4	rpoA-petD	4	AAGACTCGGAAGACTGTCCA	TGGTTGGGTATTGGAGCAAC	20	20	50.0	50.0	58.0	58.1	3.8	0.0	0.085330	172072	172992	1008-1131	0.461538	69.3
rpoA-petD_p5	rpoA-petD	5	AAGACTCGGAAGACTGTCCA	GTTTCAGTACCTGCGGGATT	20	20	50.0	50.0	58.0	57.9	3.8	21.4	0.112626	172072	172992	1128-1251	0.461538	68.3