Hotspot

psbA

IRa · 96556-97614 · 1059 bp

Back to Pedinomonas

Pi 0.49
MarkerSeek score 80.50
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
IRa
length_bp
1059
variable_sites
510
pi
0.49
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 CTGATAGCTCAGCATAGCCG AACTGGCTGAGAACCAAGTG 55.0 50.0 58.0 58.0 1855 bp 0.5 59.7
psbA_p2 CTGATAGCTCAGCATAGCCG GTGAACTGGCTGAGAACCAA 55.0 50.0 58.0 58.0 1858 bp 0.5 59.7
psbA_p3 CTGAATAGCAGCTGTCTGGG AACTGGCTGAGAACCAAGTG 55.0 50.0 58.0 58.0 1994 bp 0.5 59.4
psbA_p4 CTGAATAGCAGCTGTCTGGG GTGAACTGGCTGAGAACCAA 55.0 50.0 58.0 58.0 1997 bp 0.5 59.3
psbA_p5 CTGATAGCTCAGCATAGCCG AGTTTCTCGTGAACTGGCTG 55.0 50.0 58.0 58.1 1866 bp 0.5 58.9
psbA_p1 AACTGGCTGAGAACCAAGTG CTGATAGCTCAGCATAGCCG 50.0 55.0 58.0 58.0 1855 bp 0.5 59.7
psbA_p2 GTGAACTGGCTGAGAACCAA CTGATAGCTCAGCATAGCCG 50.0 55.0 58.0 58.0 1858 bp 0.5 59.7
psbA_p3 AACTGGCTGAGAACCAAGTG CTGAATAGCAGCTGTCTGGG 50.0 55.0 58.0 58.0 1994 bp 0.5 59.4
psbA_p4 GTGAACTGGCTGAGAACCAA CTGAATAGCAGCTGTCTGGG 50.0 55.0 58.0 58.0 1997 bp 0.5 59.3
psbA_p5 AGTTTCTCGTGAACTGGCTG CTGATAGCTCAGCATAGCCG 50.0 55.0 58.1 58.0 1866 bp 0.5 58.9