Hotspot

orf216

LSC · 57960-58610 · 651 bp

Back to Pedinomonas

Pi 0.479

MarkerSeek score 80.90

Primer available yes

Label orf216

Feature Metadata

label_name: orf216
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 264
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf216_p1	GTGGTCGCAAGTGATTTGTG	TCGGTGGTGGTACTTTAGGT	50.0	50.0	58.0	58.0	1553 bp	0.5	59.6
orf216_p2	GCTTTTCGCGGAACAGTTAC	TCGGTGGTGGTACTTTAGGT	50.0	50.0	58.1	58.0	1596 bp	0.5	59.5
orf216_p3	GTGGTCGCAAGTGATTTGTG	AGCAGCTTGTGAAGTTTGGA	50.0	45.0	58.0	57.9	1390 bp	0.5	59.3
orf216_p4	CTGAAATCGGAAACTCGGGT	TCGGTGGTGGTACTTTAGGT	50.0	50.0	57.9	58.0	1390 bp	0.5	59.1
orf216_p5	GCTTTTCGCGGAACAGTTAC	AGCAGCTTGTGAAGTTTGGA	50.0	45.0	58.1	57.9	1433 bp	0.5	59.1